Distribution
These studies are a vital part of the ADME (Absorption, Distribution, Metabolism, and Excretion) profiling in drug discovery. These studies help predict how a drug will spread throughout the body after administration, which is crucial for understanding its efficacy, potential toxicity, and dosing strategy.
Red blood cell partition
Protein binding
Red blood cell partition
| Protocol | |
|---|---|
| Incubation matrix | Blood |
| Species | As per the client request |
| Replicates | 3 |
| Concentration | 1 µM |
| Incubation temperature | 37 °C |
| % DMSO content | ≤ 1% |
| Time points | 1 hr |
| Assay controls | Chloroquine and Verapamil |
| Analysis | LC-MS/MS |
| Compound requirement | 10 mM DMSO stock 10 µL |
Depletion approach
Species
- Mouse
- Rat
- Human
Direct Approach
Species
- Rat
- Human
Protein binding
Rapid Equilibrium Dialysis (RED)
| Protocol | |
|---|---|
| Method | Qualitative or Quantitative |
| Species | As per client request |
| Incubation matrix | As per client request |
| Replicates | 2 |
| Concentration | 2 µM |
| Incubation temperature | 37 °C |
| % DMSO content | ≤ 1% |
| Time points | 0 hr and 5 hr |
| Assay controls | Carbamazepine and Warfarin |
| Analysis | LC-MS/MS |
| Compound requirement | 10 mM DMSO stock 10 µL |
Species
- Mouse
- Rat
- Dog
- Monkey
- Human
Matrix
- Blood
- Plasma
- Serum
- Human Serum Albumin (HSA)
- α1 Acid Glycoprotein (AAG)
- Cell Culture Media
- Hepatocytes
- Liver microsomes
- Tissue homogenates
- Liver | Muscle | Brain | Kidney
Ultracentrifugation (UC)
| Protocol | |
|---|---|
| Method | Qualitative or Quantitative |
| Species | As per client request |
| Incubation matrix | As per client request |
| Replicates | 2 |
| Concentration | 2 µM |
| Incubation time | 5 hr at 37 °C |
| % DMSO content | ≤ 1% |
| Time points | 0, 5 hr and 5 hr stability |
| Analysis | LC-MS/MS |
| Assay controls | Carbamazepine and Warfarin |
| Compound requirement | 10 mM DMSO stock 10 µL |
High Throughput Dialysis (HT Dialysis)
Species: Mouse, Rat, Dog, Monkey and Human
- Matrix
- Plasma
- Serum
- Human Serum Albumin (HSA)
- α1 Acid Glycoprotein (AAG)
- Cell Culture Media
Species
- Mouse
- Rat
- Dog
- Monkey
- Human
Matrix
- Blood
- Plasma
- Serum
- Liver Microsomes
- Hepatocytes
- Tissue homogenates
- Liver | Muscle | Brain | Kidney
