PHASE 0 STUDIES : PATIENTS TUMOR SLICE CULTURE (PTSC) FOR PD/EFFICACY & BIOMARKERS
A novel ex-vivo platform to evaluate multiple drug response profiles on intact human tumor fragments cultured with matched patient immune cells (mimics the human tumor-immune microenvironment)
Preservation readouts
- Metabolic : WST and secreted LDH
- Tumor morphology : H&E
- Phenotypic : Ki67 for proliferation; cleaved caspase-3 for apoptosis
Modulation readouts
- Preservation readouts + target engagement markers
- PD markers
Immune cells Readout
- Perform mIHC using lymphoid proliferation panel with H&E
- Measure key immune marker parameters
- Change in CD8+ TILS
- CD8: Treg ratio
- Change in CD4+ TILS
- Others
- Dynamic tumor imaging showing spatial rearrangement of T-cells
TUMOR SLICE CULTURE FROM TNBC PATIENTS RESONDING AND NOT RESPONDING TO 5FU-SOC
- Readout
- Functional
- Cell proliferation (Ki-67)
- Cell death (Caspase-3/Tunnel/LDH)
- Pathology (H&E)
- Biomarkers
- IHC/IF (PD and Tumor Panel)
- DNA/RNA (Mutation/RNASeq)
- Immune Panel Lipid
